The greatest crossover rate observed in pet and you will flowers Leave a comment

The greatest crossover rate observed in pet and you will flowers

A low NCO gene conversion rate

You to definitely domain shines because the an excellent recombinational wilderness. No CO or gene sales enjoy was perceived when you look at the a roughly 400 kb region close the two linked sex devotion family genes, csd and you can fem (A lot more document step one: Contour S14). I advise that, while the heterozygosity away from csd identifies brand new sex from honeybee [56,57] and you will diploid homozygotes is sterile boys, gene transformation inside domain name would-be disadvantageous. Since the gene conversion process was associated one another having CO and you may NCO situations, all the recombination might be abolished to quit homogenization. An absence of recombination in addition to pushes the 2 loci to behave due to the fact just one haplotype.

Our very own effect stands in hitting examine so you can an early on declare that found acutely highest CO rates near the csd locus. A prospective solution of the contradictory claims is the fact while in new instant vicinity of your genetics there’s absolutely no recombination, this will be counterbalanced of the surprisingly higher costs regarding covers proximal on the wasteland. A diminished solution data manage choose the greater cost regarding spans. In line with this i observe a leading height out of recombination in this new quick vicinity of your plateau (A lot more file step one: Shape S14).


All of our investigation increase the notion you to personal hymenoptera enjoys each other highest and very changeable crossing-more than rates. In fact, the pace varied between 0 and you may 197 cM/Mb whenever counted in the low-overlapping two hundred kb screen all over chromosomes (Contour 3, Figure S8 inside the Extra file step one and you may Dining table S6 inside the More document dos), suggesting this new extremely uneven delivery away from crossovers from the genome. The new shape from 37 cM/Mb, while large to possess pet, remains below one to seen for some fungi and you can protozoans, in which rates over sixty cM/Mb try said . Towards the good our studies, the latest guess means a top crossing-more speed than just observed in people bush otherwise animal.

While our study finds evidence consistent with several theoretical predictions, cause and effect are always hard to disentangle from correlation alone. For example, in principle the diversity/crossing-over coupling is also consistent with the notion that crossing over occurs preferentially in domains of high diversity and with the notion that crossing over is mutagenic . Similarly, the correlation between recombination rate and GC content is consistent with both the possibility that crossing over forces a high GC content and with the possibility that a high GC content favors increased crossing over. The conventional wisdom holds that recombination forces heteroduplexes within which mismatches require resolution. If this resolution is biased towards G and C residues (biased gene conversion), then a correlation between GC and recombination rate is expected . Previous analyses in honey bee have demonstrated fixation biases toward GC in instabang profile search high recombination parts of the genome . Evidence to support this direction for the causal arrow requires SNP analysis showing a bias to AT- > GC SNPs being fixed (or otherwise favored) compared to GC- > AT SNPs at sites of recombination. The key events here are most probably crossover-associated gene conversion events, but for these we cannot determine the direction of conversion and we are likely to be missing the majority of them. In our identified NCO events, if u is the number of AT > GC SNPs per A or T and v is the number of GC > AT SNPs per G or C, then the ratio of u/v is 1.06, which is slightly greater than the null (from stochastic simulations with 10,000 repeats: P <0.08). Being on the edge of significance, we cannot robustly say that NCO is or is not associated with GC-biased gene conversion.

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